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KMID : 0381920020320020175
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Korean Journal of Microscopy
2002 Volume.32 No. 2 p.175 ~ p.183
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Immuno-Electron Microscopic Studies on the Distribution of Dopamine and Calbindin-D28K in the Optic lobes of Cephalopods (Todarodes pacificus and Octopus minor) inhabiting the Korean waters
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Han Jong-Min
Chang Nam-Sub
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Abstract
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In this study, we carried out immunostaining and immunogold labeling with rabbit anti-dopamine (TH) and rabbit anti-calbindin-D28K to examine the characteristics and functions of the neurons that secrete neurotransmitters in optic lobes of Todarodes pacificus and Octopus minor inhabiting the Korean waters. The obtained results are as follow. In the immunostaining with anti-dopamine, only a few of the large amacrine cells in an the upper part of an outer granule cell layer and the cells forming the islands of medulla showed positive reaction in Todarodes pacificus, while 2~3 cells in the upper and middle parts of an outer granule cell layer and more than 5 cells in the islands of medulla reacted positively in Octopus minor. For the case of anti-calbindin case, 2~3 small amacrine cells in the upper portion of the outer granule cell layer and 1~2 cells which are located in the lower part of an inner granule cell layer showed positive reaction in Todarodes pacificus, while, in Octopus minor, 4 cells in the outer granule cell layer reacted positively, no immunoreactive cell being found in the inner granule cell layer. As a result of performing the immunogold labeling, relative large number (17~26) of gold particles were labeled per 0.5㎛ 2 of the cytoplasm of the cells which showed the immunoreactivity to the anti-dopamine and anti-calbindin in Todarodes pacificus, however, small number (10) of gold particles were labeled in Octopus minor, which reach only half of the number in the Todarodes pacificus.
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KEYWORD
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Anti-calbindin , Anti-dopamine , Immunogold labeling , Immunostaining
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